The potyvirus recessive resistance gene, sbm1, identifies a novel role for translation initiation factor eIF4E in cell‐to‐cell trafficking
- 14 September 2004
- journal article
- Published by Wiley in The Plant Journal
- Vol. 40 (3) , 376-385
- https://doi.org/10.1111/j.1365-313x.2004.02215.x
Abstract
Summary: From the characterization of the recessive resistance gene, sbm1, in pea we have identified the eukaryotic translation initiation factor, eIF4E, as a susceptibility factor required for infection with the Potyvirus, Pea seed‐borne mosaic virus. A functional analysis of the mode of action of the product of the dominant allele revealed a novel function for eIF4E in its support for virus movement from cell‐to‐cell, in addition to its probable support for viral RNA translation, and hence replication. Different resistance specificities in two independent pea lines were explained by different mutations in eIF4E. On the modelled structure of eIF4E the coding changes were in both cases lying in and around the structural pocket involved in binding the 5′‐m7G cap of eukaryotic mRNAs. Protein expression and cap‐binding analysis showed that eIF4E encoded by a resistant plant could not bind to m7G‐Sepharose, a result which may point to functional redundancy between eIF4E and the paralogous eIF(iso)4E in resistant peas. These observations, together with related findings for other potyvirus recessive resistances, provide a more complete picture of the potyvirus life cycle.Keywords
This publication has 40 references indexed in Scilit:
- Advances in understanding recessive resistance to plant virusesMolecular Plant Pathology, 2004
- Identification of markers tightly linked to sbm recessive genes for resistance to Pea seed-borne mosaic virusTheoretical and Applied Genetics, 2004
- The Arabidopsis eukaryotic initiation factor (iso)4E is dispensable for plant growth but required for susceptibility to potyvirusesThe Plant Journal, 2002
- Cap-Independent Translation Conferred by the 5′ Leader of Tobacco Etch Virus Is Eukaryotic Initiation Factor 4G DependentJournal of Virology, 2001
- Mutations in Pea seedborne mosaic virus Genome-Linked Protein VPg Alter Pathotype-Specific Virulence in Pisum sativumMolecular Plant-Microbe Interactions®, 2001
- Stabilization of Eukaryotic Initiation Factor 4E Binding to the mRNA 5′-Cap by Domains of eIF4GPublished by Elsevier ,2000
- Simple hand-held devices for the efficient infection of plants with viral-encoding constructs by particle bombardmentJournal of Virological Methods, 1997
- Impaired Cell-to-Cell Movement of Potato Virus Y in Pepper Plants Carrying the ya(pr21)Resistance GeneMolecular Plant-Microbe Interactions®, 1996
- Function of the p86 subunit of eukaryotic initiation factor (iso)4F as a microtubule-associated protein in plant cells.Proceedings of the National Academy of Sciences, 1995
- Production of Transgenic Rice (Oryza Sativa L.) Plants from Agronomically Important Indica and Japonica Varieties via Electric Discharge Particle Acceleration of Exogenous DNA into Immature Zygotic EmbryosBio/Technology, 1991