CHARACTERIZATION OF WATER-SOLUBLE ORGANIC SUBSTANCES FROM A TYPIC DYSTROCHREPT UNDER SPRUCE USING GPC, IR, 1H NMR, AND 13C NMR SPECTROSCOPY

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Abstract
Water-soluble organic matter obtained from each horizon, litter layer through the B horizon, from a Typic Dystrochrept under a spruce (Picea abies) forest was fractionated on Sephadex G-25 (medium). Fractionation patterns and spectroscopic data suggest some typical differences within horizons as well as among horizons and between seasons. Dissimilar carbohydrate structures constitute the principal differences. The litter layer appears to have more aliphatic hydrogen moieties associated with these water-soluble substances than those of the deeper soil horizons. This is in contrast to the behavior of total alkyl C in the same horizons, which increases from L to Oh, Aeh, and Bhs, indicating changes in the structural habitus, probably due to higher polymerization during humification.