The Estimation of the Concentration and Equilibrium Constant of Anti-D

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Abstract
Summary A method is described for estimating the concentration and equilibrium constant of the blood group antibody, anti-D. The principle of the method is based on the fact that the reaction between anti-D and red cells is reversible and obeys the law of mass action. D-positive red cells at five different concentrations were added to aliquots of antisera containing anti-D and the reaction allowed to come into equilibrium. The amount of anti-D bound to the red cells in each case was estimated using 125I-labelled anti-γ-globulin. The results of these estimates were then analysed according to two derivations of the law of mass action. Twenty-one examples of antisera containing anti-D were examined and the estimated range of values was as follows: concentration, 0 3–7.2 μg/ml; equilibrium constant, 1.4 × 107 to 1.2 × 109 1/mole; index of heterogeneity, a = 0.6–0.9. Keywords Equilibrium Constant Mass Action Heterogeneity Index Dilute Antiserum Mass Action Equation These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.