Comparative methods for inducing and measuring non-shivering thermogenesis in newborn lambs

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Abstract
Thirty-one newborn lambs were allocated to four treatments involving subcutaneous or slowly administered intravenous injections of noradrenaline (a natural predominantly a-agonist) or isoprenaline (a synthetic pure ($-agonist). The thermogenic effect of these treatments, attributed to brown adipose tissue, was assessed by changes in metabolic rate and rectal temperature. The objective was to define the optimum treatment for studying genetic variation between lambs in the thermogenic activity of brown adipose tissue. After all treatments, metabolic rate increased significantly by about 7 W/kg to a peak averaging 2·3 times the resting metabolic rate. Rectal temperature increased significantly by about 1°C. Both drugs administered at 150 [ig/kg were similar in potency with noradrenaline producing slightly, but not significantly, greater responses; subcutaneous injections gave significantly larger and more prolonged responses than intravenous injections. Subcutaneous isoprenaline produced the longest sustained response with peak metabolism difficult to define. Subcutaneous noradrenaline injections provided the simplest satisfactory treatment for large scale experiments.