Characterization of Endopolygalacturonase Produced byRhizoctonia solaniin Culture and During Infection of Cotton Seedlings

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Abstract
An isolate of anastomosis group 4 of R. solani produced endopolygalacturonase (endoPG) in culture in response to cotton seed exudates and in infected cotton seedlings 18 h after inoculation. No other depolymerizing type of pectic enzyme was detected during fungal growth under these conditions. EndoPG from these sources had pH optima of 5.2 and MW of .apprx. 42,000 daltons. Enzyme preparations from culture and infected seedlings readily macerated cotton hypocotyl sections. Isoelectric focusing techniques showed that the endoPG produced by R. solani in response to seed exudates had a single isoelectric point (pI) of 7.1 whereas endoPG from infected hypocotyls had a major peak at pI 7.8 and a minor peak at 7.1. Differences in elution patterns were observed when preparations from the 2 sources were purified by ion exchange chromatography. This study suggests that R. solani produces different forms of endoPG and that the ionic properties of the predominant form produced during pathogenesis differ from those of the single-peak form produced in culture.

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