On different methods available for measuring the area of gill secondary lamellae of fishes

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Abstract
Advantages and disadvantages of methods used to estimate area of individual secondary lamellae are discussed. Particular attention is given to explain the method which regards filaments as triangles and lamellae as rectangles. Dangers of some injection methods assuming that the whole lamellar microcirculation is equally involved in gas exchange are also emphasized. It is concluded that there is a need for comparisons of results when different methods are applied to the same material.