A GC-MS method for determination of .DELTA.9-tetrahydrocannabinol-11-oic acid in human urine.
- 1 January 1990
- journal article
- research article
- Published by Pharmaceutical Society of Japan in Eisei kagaku
- Vol. 36 (2) , 149-152
- https://doi.org/10.1248/jhs1956.36.149
Abstract
The GC-MS and TLC methods for the determination and identification of Δ9-tetrahydrocannabinol-11-oic acid, a major urinary metabolite of Δ9-tetrahydrocannabinol which is one of main constituents of marihuana, were established. Δ9-Tetrahydrocannabinol-11-oic acid was detected on TLC by using Fast Blue BB salt as a color reagent. The metabolite was determined as trimethylsilyl derivative of its methyl ester by using electron impact ionization GC-MS and 5'-nor-Δ8-tetrahydrocannabinol-4'-oic acid as an internal standard. The assay provides excellent sensitivity, reproducibility and specificity with detection limit of 1 ng/ml human urine.Keywords
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