Characterization of the Sulfated Monosialosyltriglycosylceramide from Bovine Gastric Muscosa

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Abstract
A sulfated monosialosyltriglycosylceramide was isolated from the lipid extract of bovine gastric mucosa in a yield of 2.52 μM/kg of wet tissue. The isolation procedure involved extraction of lipids with 0.4M sodium acetate in methanol/chloroform/water, alkaline methanolysis, column fractionation on DEAE‐Sephadex and silicic acid, and thin‐layer chromatography. Based on the results of partial acid hydrolysis, sequential degradation with specific glycosidases, periodate oxidation, and methylation analysis of the native and partially degraded compund, the structure of this glycolipid is proposed to be: GalNAcβ1→8Sia→2→3)Galβ→4Glcl→1Cer. The sialic acid of this glycolipid consisted of N‐acetylneuraminie acid and N‐glycoloylneuraminic acid, with the former being predominant component (71%) of the mixture.