Cetyl Pyridinium Chloride

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Abstract
Cetyl pyridinium chloride was used as the test compound and a virulent strain of Salmonella typhimurium as the test organism. White mice (20-25 g.) were inoculated intra-perit. with 0.25 ml. of the germicide-bacteria mixture plus 0.25 ml. of 3% sterile mucin after the bacteria had been treated with the various germicide dilutions for 5- and 10-min. intervals. 5 hrs. after inoculation specimens of heart blood and peritoneal fluid were obtained aseptically, and cultured in FDA broth. The following day positive cultures were streaked on Bacto SS agar and bismuth sulfite agar. Presence of organisms of the genus Salmonella was confirmed on Kligler''s iron agar slants. Recovery of the viable pathogen from the exptl. animal indicated lack of bactericidal action, conversely failure to recover the organism indicated bactericidal effect. Ten tests in vivo gave an average killing dilution of 1:45,000, and 10 in vitro tests gave an average killing dilution of 1:58,000. The in vivo and in vitro results compared favorably and no reversal of germicidal action by body fluids was indicated. The in vivo method of evaluation provides a useful comparative test for this cationic germicide under conditions similar to those in medical practice.

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