Chromatin very small angle neutron scattering: further evidence for a 30 nm diameter super coil in dilute solutions
Open Access
- 1 January 1977
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 4 (7) , 2275-2282
- https://doi.org/10.1093/nar/4.7.2275
Abstract
Intact chromatin, chromatin minus histone H1, and nuclease digestion fragments have been studied by very small angle neutron scattering. The results are not consistent with a straight chain of nucleosomes and require the presence of a higher order coiling in monovalent salt solutions. The data are interpretable by a structure having a cross section radius of gyration of 8.5 ± 1 nm, which suggests an outer diameter for a coil of nucleosomes of 27 ± 3 nm.Keywords
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