IMMUNOHISTOCHEMICAL DEMONSTRATION OF HYALURONIDASE IN THE SHEEP TESTIS

Abstract
The localization of hyaluronidase in the sheep testis was investigated by use of the peroxidase-labeled antibody method on the light microscopic and EM levels. The antigen was recognized in the acrosome of the spermatid and spermatozoon. In EM observation, the antigen in the spermatid was concentrated in the acrosomal cap, but was not recognized in the acrosomal collar. In the spermatozoon, the antigen was recognized homogeneously in the acrosomal cap and in the acrosomal collar. Hyaluronidase localized in the acrosomal cap evidently spreads into the acrosomal collar region as spermatogenesis proceeds from spermatid to spermatozoon.

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