PCR with degenerate primers containing deoxyinosine fails with Pfu DNA polymerase.
Open Access
- 1 May 1993
- journal article
- research article
- Published by Cold Spring Harbor Laboratory in Genome Research
- Vol. 2 (4) , 346-347
- https://doi.org/10.1101/gr.2.4.346
Abstract
An international, peer-reviewed genome sciences journal featuring outstanding original research that offers novel insights into the biology of all organismsThis publication has 6 references indexed in Scilit:
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- DEGENERATE PRIMERS FOR DNA AMPLIFICATIONPublished by Elsevier ,1990
- PCR amplification of anEscherichia coligene using mixed primers containing deoxyinosine at ambiguous positions in degenerate amino acid codonsNucleic Acids Research, 1990
- Highly degenerate, inosine-containing primers specifically amplify rare cDNA using the polymerase chain reactionNucleic Acids Research, 1988