Glyphosate tolerant flax plants from Agrobacterium mediated gene transfer
- 1 June 1988
- journal article
- research article
- Published by Springer Nature in Plant Cell Reports
- Vol. 7 (4) , 281-284
- https://doi.org/10.1007/bf00272543
Abstract
Agrobacterium tumefaciens carrying a disarmed Ti-plasmid vector containing a chimeric NPT-II gene and a glyphosate resistance plant-derived 5-enolpyruvylshikimate-3-phosphate synthase gene was used to transform flax hypocotyl tissues. Transformed shoots could be regenerated from the inoculated tissue and were proven to be transgenic by the combination of leaf callus assays, nopaline assays and progeny tests. Co-segregation was observed in the progeny for kanamycin and glyphosate resistance.This publication has 15 references indexed in Scilit:
- The herbicide glyphosate is a potent inhibitor of 5-enolpyruvylshikimic acid-3-phosphate synthasePublished by Elsevier ,2004
- Transformation of Brassica napus with Agrobacterium tumefaciens based vectorsPlant Cell Reports, 1987
- Genetic transformation of flax (Linum usitatissimum) by Agrobacterium tumefaciens: regeneration of transformed shoots via a callus phasePlant Cell Reports, 1987
- Agrobacterium-Mediated Plant Transformation and its Further Applications to Plant BiologyAnnual Review of Plant Physiology, 1987
- Genetically Transformed Cotton (Gossypium Hirsutum L.) PlantsBio/Technology, 1987
- Engineering Herbicide Tolerance in Transgenic PlantsScience, 1986
- Expression in plants of a mutant aroA gene from Salmonella typhimurium confers tolerance to glyphosateNature, 1985
- A Simple and General Method for Transferring Genes into PlantsScience, 1985
- Tissue Culture, Protoplasts, and Morphogenesis in FlaxBotanical Gazette, 1976
- A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue CulturesPhysiologia Plantarum, 1962