Marked Effect of Cations on the Separation of Spinach Thylakoid Chlorophyll-Proteins on Polyacrylainide Gel Electrophoresis at 0°C in the Presence of Alkanolammonium Dodecyl Sulfates1
- 1 April 1986
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 99 (5) , 1545-1548
- https://doi.org/10.1093/oxfordjournals.jbchem.a135626
Abstract
The separation of spinach thylakoid chlorophyll-proteins on polyacrylamide gel electrophoresis at 0°C in the presence of dodecyl sulfate is markedly influenced by the kind of surfactant cation in the media used for solubilization and electrophoresis. The mode of separation can thus be modulated through the cation selection. Three kinds of alkanolammonium dodecylsulfates were tested and their abilities as to the dissociation of the molecular assemblies of chlorophyll-proteins were found to decrease in the following order: tris(hydroxymethyl)methylammonium, triethanolammonium and triisopropanolammonium. Comparison of the electrophoretic patterns obtained with different kinds of cation may help clarify the hierarchy of the molecular assemblies of chlorophyll-proteins.This publication has 3 references indexed in Scilit:
- Lithium dodecyl sulfate/polyacrylamide gel electrophoresis of thylakoid membranes at 4°C: Characterizations of two additional chlorophyll a-protein complexesProceedings of the National Academy of Sciences, 1979
- Identification of chloroplast membrane peptides with subunits of coupling factor and ribulose-1,5 diphosphate carboxylaseArchives of Biochemistry and Biophysics, 1976
- Two pigment proteins in spinach chloroplastsBiochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis, 1966