Presynaptic calcium stores underlie large-amplitude miniature IPSCs and spontaneous calcium transients
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Open Access
- 1 December 2000
- journal article
- research article
- Published by Springer Nature in Nature Neuroscience
- Vol. 3 (12) , 1256-1265
- https://doi.org/10.1038/81781
Abstract
The cellular mechanisms responsible for large miniature currents in some brain synapses remain undefined. In Purkinje cells, we found that large-amplitude miniature inhibitory postsynaptic currents (mIPSCs) were inhibited by ryanodine or by long-term removal of extracellular Ca2+. Two-photon Ca2+ imaging revealed random, ryanodine-sensitive intracellular Ca2+ transients, spatially constrained at putative presynaptic terminals. At high concentration, ryanodine decreased action-potential-evoked rises in intracellular Ca2+. Immuno-localization showed ryanodine receptors in these terminals. Our data suggest that large mIPSCs are multivesicular events regulated by Ca2+ release from ryanodine-sensitive presynaptic Ca2+ stores.Keywords
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