STUDIES ON PRODUCTION OF BIOLOGICALLY ACTIVE SUBSTANCES WHICH INHIBIT CELL MIGRATION IN SUPERNATANTS AND EXTRACTS OF HYPERSENSITIVE LYMPHOID CELLS INCUBATED WITH SPECIFIC ANTIGEN IN VITRO

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  • 1 January 1968
    • journal article
    • research article
    • Vol. 15  (1) , 1-+
Abstract
When lymphoid cells from hypersensitive rabbits are incubated with antigen, bi ologically active substances are formed and released which are capable of inhibiting the migration of normal non-sensitized mesenchymal cells, fa the present paper some basic parameters of their production were determined. These substances were regularly obtained after 6 and 18 hr. incubation, but not after 2 hr. Under more favorable cultivation conditions (lower density of lymphocyte suspension) an increased activity in the cell extracts as compared with the super-natants was observed. Another critical factor in the production of these substances is the quantity of antigen added. Ten micrograms of PPD [purified protein derivative] leads to the production and liberation of a highly effective Substance. A lower dose of antigen results in the liberation of a substance into the supernatant which by itself is almost inactive, but becomes more active when more antigen is added. The efficiency of the released substances was determined by serial dilution. The inhibiting activity was maintained at 1:5 and 1:20 and sometimes at 1:100 dilutions.