REFINED MAP OF THE HISG GENE OF SALMONELLA-TYPHIMURIUM

  • 1 January 1979
    • journal article
    • research article
    • Vol. 92  (1) , 17-26
Abstract
The hisG gene is the most operator-proximal structural gene of the histidine operon; it encodes the feedback-inhibitable 1st enzyme of the biosynthetic pathway. Previously, hisG mutants were mapped into 7 intervals defined by the available deletion mutations having endpoints in the hisG gene. The map was refined using over 60 new deletion mutants. The new map divides the gene into 40 deletion intervals, which average approximately 30 base pairs in length. The map was used to analyze the distribution of insertion sites for the transposable element Tn10 and permitted conclusions on the distribution of duplication endpoints. The map promises to be useful in analysis of his regulation and, more particularly, in the determination of the possible role of the hisG enzyme in this mechanism.