Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assay for Glycyrrhizin and Its Aglicon, Glycyrrketic Acid
- 1 February 1994
- journal article
- research article
- Published by Taylor & Francis in Journal of Immunoassay
- Vol. 15 (1) , 21-34
- https://doi.org/10.1080/15321819408009569
Abstract
Monoclonal antibodies (MoAbs) specific for glycyrrhetic acid (GA) were prepared and characterized. Obtained MoAbs (AGA-1, AGA-3, and AGA-6) reacted with GA dose-dependently, but not with glycyrrhizin (GL), carbenoxolone, and steroids. Next, an enzyme-linked immunosorbent assay (ELISA) system using AGA-1 was established. The standard curve showed good linearity between 0.01 and 1000 ng/ml of GA, and the detection limit was 5 pg/ml. Recovery, and intra- and interassay variations of this assay system was satisfactory. GL was also measurable quantitatively after acid-hydrolysis of the samples. The developed ELISA system would be useful to determine GL and GA in biological samples or drugs as an alternative method to high performance liquid chromatography (HPLC).Keywords
This publication has 18 references indexed in Scilit:
- The In Vitro Effects of Glycyrrhizin and the Derivatives of Glycyrrhetinic Acid on the Activity of cAMP-Dependent Protein Kinase and Phosphorylation of Cellular Polypeptide by the Kinase from Ehrlich Ascites Tumor Cells.The Tohoku Journal of Experimental Medicine, 1991
- Characterization of glycyrrhizin-binding protein kinase from the crude membrane fraction of rat liverBiochemical and Biophysical Research Communications, 1990
- Inhibitory effect of glycyrrhizin on polypeptide phosporylation by polypeptide-dependent protein kinase (Kinase P) invitroBiochemical and Biophysical Research Communications, 1988
- Mechanism of inhibitory effect of glycyrrhizin on replication of human immunodeficiency virus (HIV)Antiviral Research, 1988
- Studies on the enzyme immunoassay of bio-active constituents contained in oriental medicinal drugs. IV. Enzyme immunoassay of glycyrrhetic acid.CHEMICAL & PHARMACEUTICAL BULLETIN, 1988
- Preparation of (3.ALPHA.-3H)3.BETA.-hydroxy-18.BETA.- and 3.ALPHA.-hydroxy-18(.BETA. and .ALPHA.)-glycyrrhetic acid and radioimmunoassay of glycyrrhetic acid.CHEMICAL & PHARMACEUTICAL BULLETIN, 1988
- Inhibitory effect of glycyrrhizin on the in vitro infectivity and cytopathic activity of the human immunodeficiency virus [HIV (HTLV-III/LAV)]Antiviral Research, 1987
- Glycyrrhetylamino acids: Synthesis and application to enzyme immunoassay for glycyrrhetic acid.CHEMICAL & PHARMACEUTICAL BULLETIN, 1981
- CLINICAL TRIAL OF A TRITERPENOID LIQUORICE COMPOUND IN GASTRIC AND DUODENAL ULCERThe Lancet, 1962
- THE ANTI-INFLAMMATORY ACTIVITY OF GLYCYRRHETINIC ACID AND DERIVATIVESJournal of Pharmacy and Pharmacology, 1958