Adaptation-like response to the chemical induction of sister chromatid exchanges in human lymphocytes
- 1 May 1986
- journal article
- research article
- Published by Springer Nature in Human Genetics
- Vol. 73 (1) , 81-85
- https://doi.org/10.1007/bf00292670
Abstract
Experiments have been performed to determine whether human lymphocytes in primary cultures can show an “adaptive” response to the induction of cellular lesions (manifested as a production of sister chromatid exchanges, SCEs) as previously found in bacteria and established human and mammalian cell lines. Human lymphocytes were pretreated with various subtoxic concentrations (5–50ng/ml) of N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) once every 6h for 72h, and subsequently challenged by a high dose (4μg/ml) of MNNG. The lymphocytes in MNNG-challenged cultures had the lowest frequency of SCEs when pretreated with 10ng/ml MNNG. Further cross-resistance study revealed that repeaied pretreatments of lymphocytes with 10ng/ml MNNG for 72h can render the cells resistant to the induction of SCEs by the following challenge with a high dose of MNNG, but not of mitomycin C or ethyl nitrosourea. The data also suggest variations in the degree of the adaptation-like response among individuals.Keywords
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