Plant regeneration from cell cultures of Lotus corniculatus and the selection and characterization of 2,4-D tolerant cell lines
- 15 May 1980
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Botany
- Vol. 58 (10) , 1205-1209
- https://doi.org/10.1139/b80-150
Abstract
The isolation and characterization of a rapidly growing, friable, green, 2,4-D tolerant callus line of birdsfoot trefoil (Lotus corniculatus cv. Leo) is described. Differences in cell morphology, growth rate, totipotency, and 2,4-D tolerance were examined between selected and control callus stocks. Plants were regenerated from both the 2,4-D tolerant callus and from a number of callus lines which had been exposed to high 2,4-D levels. The selected plant populations are being maintained and increased by shoot tip culture.Keywords
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