Study of the reaction of nitrosobenzene with haemoglobine

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Abstract
During the reaction of haemoglobine with nitrosobenzene, the latter was determined polarographically. Nitrosobenzene bound by coordination to the divalent Fe atom of haemoglobine was polarographically inactive; it was set free from this complex by oxidation of the iron with ferricyanide and then determined. At a molar ratio of nitrosobenzene to haemoglobine of 4 : 1, at which the molar ratio of iron to nitrosobenzene is 1 : 1, 0.9 mol of nitrosobenzene is bound by coordination to the iron while 0.1 mol is reduced by SH groups to azo- and azoxybenzene. After the SH groups of haemoglobine had been blocked by cyanoethylation or oxidation, nitrosobenzene was bound to iron in a stoichiometric ratio. Accordingly, the conditions for the polarographic determination of nitrosobenzene after oxidative splitting of the complex with haemoglobine are defined.

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