uORFs with unusual translational start codons autoregulate expression of eukaryotic ornithine decarboxylase homologs
- 22 July 2008
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 105 (29) , 10079-10084
- https://doi.org/10.1073/pnas.0801590105
Abstract
In a minority of eukaryotic mRNAs, a small functional upstream ORF (uORF), often performing a regulatory role, precedes the translation start site for the main product(s). Here, conserved uORFs in numerous ornithine decarboxylase homologs are identified from yeast to mammals. Most have noncanonical evolutionarily conserved start codons, the main one being AUU, which has not been known as an initiator for eukaryotic chromosomal genes. The AUG-less uORF present in mouse antizyme inhibitor, one of the ornithine decarboxylase homologs in mammals, mediates polyamine-induced repression of the downstream main ORF. This repression is part of an autoregulatory circuit, and one of its sensors is the AUU codon, which suggests that translation initiation codon identity is likely used for regulation in eukaryotes.Keywords
This publication has 47 references indexed in Scilit:
- The mechanism of an exceptional case of reinitiation after translation of a long ORF reveals why such events do not generally occur in mammalian mRNA translationGenes & Development, 2007
- Identification of novel conserved peptide uORF homology groups in Arabidopsis and rice reveals ancient eukaryotic origin of select groups and preferential association with transcription factor-encoding genesBMC Biology, 2007
- Dissociation of eIF1 from the 40S ribosomal subunit is a key step in start codon selection in vivoGenes & Development, 2007
- The Eukaryotic Translation Initiation Factors eIF1 and eIF1A Induce an Open Conformation of the 40S RibosomeMolecular Cell, 2007
- Neuronal growth and survival mediated by eIF5A, a polyamine-modified translation initiation factorProceedings of the National Academy of Sciences, 2007
- Ribosomal frameshifting in decoding antizyme mRNAs from yeast and protists to humans: close to 300 cases reveal remarkable diversity despite underlying conservationNucleic Acids Research, 2007
- Downstream control of upstream open reading frames: Figure 1.Genes & Development, 2006
- Targeting ornithine decarboxylase in Myc-induced lymphomagenesis prevents tumor formationCancer Cell, 2005
- uAUG and uORFs in human and rodent 5′untranslated mRNAsGene, 2005
- Interactions of Eukaryotic Translation Initiation Factor 3 (eIF3) Subunit NIP1/c with eIF1 and eIF5 Promote Preinitiation Complex Assembly and Regulate Start Codon SelectionMolecular and Cellular Biology, 2004