Anti-BCL2 Phosphorothioate G3139 Clinical Pharmacology: Plasma Levels During Contwous Subcutaneous Infusion by HPLC Assay
- 1 July 1997
- journal article
- Published by Taylor & Francis in Nucleosides and Nucleotides
- Vol. 16 (7) , 1683-1687
- https://doi.org/10.1080/07328319708006255
Abstract
An HPLC assay has been developed to determine plasma levels of G3139 - a 18mer phosphorothioate oligonucleotide currently in Phase I clinical studies. The assay utilizes anion exchange microchromatography, aqueous LiBr gradient elution, and UV absorbance detection. Minimum sensitivity of approximately 0.2 μg/ml plasma, or 35 nM of G3139, has been achieved. Analysis of preliminary clinical samples indicates subcutaneous infusion of G3139 at 2 mg/kg/day gives rise to steady state plasma levels of 1–2 μg/ml.Keywords
This publication has 1 reference indexed in Scilit:
- Quantitative analysis of phosphorothioate oligonucleotides in biological fluids using direct injection fast anion-exchange chromatography and capillary gel electrophoresisJournal of Chromatography B: Biomedical Sciences and Applications, 1994