Exclusive formation of all-trans-phytoene by a colorless mutant of Halobacterium halobium
- 1 August 1980
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 26 (8) , 1011-1014
- https://doi.org/10.1139/m80-171
Abstract
A spontaneously occurring Halobacterium halobium mutant (strain W5002-1) was isolated which accumulated phytoene as the only C40 polyene. Its configuration was established as all-trans (7,8,11,12,7′,8′, 11′, 12′,-octahydro-ψ,ψ-carotene) by its chromatographic and spectral properties. Neither bacterioruberins (C50) nor retinal (C20) could be detected in this mutant strain. The biosynthetic pattern of isoprenoid compounds in this mutant strain confirms for the first time our conjecture that spontaneously arising H. halobium mutants can be isolated with defects in the carotenoid pathway and lends further support to our previous hypothesis suggesting all-trans-phytoene to be the precursor for more unsaturated carotenoids in halobacteria.This publication has 4 references indexed in Scilit:
- Occurrence of indole in cells of extremely halophilic bacteriaCanadian Journal of Microbiology, 1977
- Enzymatic synthesis of C40 carotenes by cell-free preparation from Halobacterium cutirubrumCanadian Journal of Biochemistry, 1976
- The configuration of phytoeneArchives of Biochemistry and Biophysics, 1965
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951