The Culture of Excised Tissue 3Lilium speciosumThun

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Abstract
Explants from the bulb scales of Lilium speciosum were cultured in vitro, where they proliferated and differentiated to regenerate bulblets in 15–16 weeks. Morphologically, regeneration takes place from mesophyll tissue, buds arising from more superficial layers, and roots from deeper layers. The regenerative capacity of explants has been shown to be seasonal and of a localized nature, basal explants regenerating freely, apical explants never regenerating. The seasonal response may be correlated with the vegetative state of the plant at the time of excision.