Simplified Co-Purification of Vascular Smooth Muscle Calponin and Caldesmon1
- 1 April 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 107 (4) , 507-509
- https://doi.org/10.1093/oxfordjournals.jbchem.a123075
Abstract
A method for the co-purification of calponin and caldesmon from bovine aorta smooth muscle was established involving heat treatment, ammonium sulfate fractionation (0–30 and 30–50%), CM52 column chromatography, and Ultrogel AcA44 gel filtration. The yields of calponin and caldesmon from 200 g of smooth muscle were 17 and 32 mg, respectively. This simplified, fast, and efficient method for the purification of calponin and caldesmon constitutes a useful tool for studying thin filament-linked regulation of smooth muscle contraction.This publication has 8 references indexed in Scilit:
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