Differential Modulatory Effects of Cholera Toxin and Pertussis Toxin on Pain Behavior Induced by TNF-a, lnterleukin-1β and Interferon- Injected Intrathecally

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Abstract
The present study was designed to characterize the possible roles of spinally located cholera toxin (CTX)- and pertussis toxin (PTX)-sensitive G-proteins in pro-inflammatory cytokine induced pain behaviors. Intrathecal injection of tumor necrosis factor-a (TNF-a; 100 pg), inter-leukin-1β (IL-1β; 100 pg) and interferon-γ (INF-γ; 100 pg) showed pain behavior. Intrathecal pretreatment with CTX (0.05, 0.1 and 0.5 mg) attenuated pain behavior induced by TNF-α and INF-γ administered intrathecally. But intrathecal pretreatment with CTX (0.05, 0.1 and 0.5 μg) did not attenuate pain behavior induced by IL-1β. On the other hand, intrathecal pretreatment with PTX further increased the pain behavior induced by TNF-a and IL-1 β administered intrathecally, especially at the dose of 0.5 μg. But intrathecal pretreatment with PTX did not affect pain behavior induced by INF-γ.Our results suggest that, at the spinal cord level, CTX- and PTX-sensitive G-proteins appear to play important roles in modulating pain behavior induced by pro-inflammatory cytokines administered spinally. Furthermore, TNF-α, IL-1 β and INF-γ administered spinally appear to produce pain behavior by different mechanisms.

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