Acid-soluble Nucleotides Analysis of BovineCoronary Artery and Aorta

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Abstract
In order to solve a problem that there is the different predilection of atherosclerosis depending on location of artery and/or race, the studies on energy-metabolism of the bovine artery were undertaken. In a previous paper, it was reported that oxygen uptake, the incor-poration of Pi32 into the adenine nucleotides, the content of DNA, and the nucleotide content of the sliced coronary artery of cattle were almost twice of those of aorta. In this paper, column chromatographic analyses of the nucleotides and nucleosides of the sliced coronary artery and aorta of cattle were studied and compared with those of bovine myocardium and rabbit skeletal muscle. And the effects of incubation for 60min. at 37°C were also investigated. The contents of nucleotides and nucleosides of the coronary artery were 1.5 and 0.2 folds of those in aorta and myocardium respectively, and approximately 90% of nucleotides were adenine nucleotides. During the incubation, approximately 40% of nucleotides and nucleosides were released into the reaction medium. Approximately 60 to 70% of the released nucleotides and nucleosides were Hypoxanthine in the coronary artery.

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