Detection of IgM Antibodies to Cytomegalovirus (CMV) Using an Enzyme-labelled Antigen (ELA)
- 1 September 1980
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 50 (1) , 59-68
- https://doi.org/10.1099/0022-1317-50-1-59
Abstract
We have applied a peroxidase enzyme-labelled antigen (ELA) for the detection of IgM antibodies to cytomegalovirus: microtitre plates were coated with anti-IgM immunoglobulin. The IgM fraction of human serum was selectively bound to the precoated plates and the virus-specific IgM antibody was then detected by the enzyme-labelled antigen. A very efficient technique for the labelling of virus antigen is described. The IgM antibody was detected simply and specifically, Rheumatoid factor IgM did not interfere with this test.This publication has 7 references indexed in Scilit:
- A Solid-phase Immunosorbent Technique for the Rapid Detection of Rubella IgM by Haemagglutination InhibitionJournal of General Virology, 1979
- A Solid-Phase Radioimmunoassay for Detection of IgM Antibodies to Hepatitis A VirusThe Journal of Infectious Diseases, 1979
- Antigen requirements, sensitivity, and specificity of enzyme immunoassays for measles and rubella viral antibodiesJournal of Clinical Microbiology, 1979
- Selective detection of IgM-antibody against core antigen of the hepatitis B virus by a modified enzyme immune assayJournal of Medical Virology, 1979
- Improved Detection of Virus-Specific IgM Antibodies. Elimination of Non-Specific IgM BindingJournal of General Virology, 1978
- Solid-phase enzyme immunoassay for immunoglobulin M antibodies to cytomegalovirusJournal of Clinical Microbiology, 1977
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951