Lipid requirements for cytochrome c oxidase activity

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Abstract
Beef heart mitochondria cytochrome c oxidase depleted of endogenous lipid by detergent exchange was reconstituted into vesicles with synthetic lipids of known head group and fatty acid composition, and enzymic activities were measured. No evidence for head group specificity was found. The enzyme requires the fluid environment provided by unsaturated fatty acids. The state of dispersion of the enzyme was found to affect activities regenerated in reconstitution studies. Highest activities were obtained using lysolecithin containing an oleoyl fatty acid as the lipid component.