Raman study of reduced nicotinamide adenine dinucleotide bound to liver alcohol dehydrogenase
- 18 December 1984
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 23 (26) , 6480-6483
- https://doi.org/10.1021/bi00321a032
Abstract
The first Raman spectra of NADH when bound to an enzymatic active site, that of liver alcohol dehydrogenase (LADH), is reported. This was obtained by subtracting the Raman spectrum of LADH from that of the binary LADH/NADH complex. There are significant changes in the spectrum of bound NADH as compared to that in solution. Both the nicotinamide moiety and the adenine moiety are involved in the binding. At least one of the 2 NH2 moieties of NADH also participates.This publication has 1 reference indexed in Scilit:
- Structure of horse liver alcohol dehydrogenaseArchives of Biochemistry and Biophysics, 1965