Platelet Preservation

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Abstract
Methods (2) were used for the preparation of platelet concentrates from fresh and overnight-stored human ACD [acid citrate dextrose] blood. In a 2-step method, prostaglandin E1 was added to platelet rich plasma and the mixture was centrifuged to obtain platelet concentrate. The IBM 2991 Blood Cell Processor was used in a 1-step method. The concentrates were stored in the frozen state. Concentrates prepared with the 2-step method had a higher platelet recovery and a lower leukocyte contamination than did concentrates prepared with the 1-step method. Based on serotonin uptake velocity, response to hypotonic stress and available platelet factor 3, no essential differences were observed between platelet concentrates prepared from fresh and overnight-stored blood. Concentrates prepared with the 1-step had a higher serotonin uptake velocity than those prepared with the 2-step method. This did not result in a better recovery after cryopreservation.