The histamine H3 receptor agonistNα-methylhistamine produced byHelicobacter pylori does not alter somatostatin release from cultured rabbit fundic D-cells

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Abstract
Background—The mechanisms underlying the suppression of somatostatin dependent reflexes in Helicobacter pylori infection are not fully determined. The H pyloriproduct Nα-methylhistamine and inflammatory mediators such as tumour necrosis factor-α (TNF-α) may be responsible for the alterations in somatostatin release.Aims—To examine the effect of Nα-methylhistamine on somatostatin release from cultured somatostatin-secreting D-cells.Methods—Rabbit fundic D-cells were obtained by collagenase-EDTA digestion and enriched by centrifugal elutriation and cultured for 40 hours. The effects ofNα-methylhistamine on somatostatin release soon after stimulation (two hours) and after more prolonged exposure (24 hours) were assessed.Results—Nα-Methylhistamine (1 nM–1 μM) had no effect on basal or carbachol or adrenaline stimulated release over two hours. Similarly with prolonged exposure no effect on somatostatin cell content or release was identified. In contrast, TNF-α (24 hours) led to a dose dependent fall in both somatostatin content and release.Conclusions—Nα-Methylhistamine had no direct inhibitory effects on D-cells, but TNF-α both significantly reduced the cellular content and inhibited release. Inflammatory cytokines, rather thanNα-methylhistamine, are therefore likely to be responsible for directly inhibiting D-cell function in H pylori infection.