A Restriction Enzyme–Based Assay to Distinguish Between Avian Hemosporidians
- 1 June 2005
- journal article
- Published by American Society of Parasitologists in Journal of Parasitology
- Vol. 91 (3) , 683-685
- https://doi.org/10.1645/ge-3412rn
Abstract
We describe a reliable and relatively inexpensive method for detecting and differentiating between the commonly studied avian blood parasite genera Haemoproteus, Plasmodium, and Leucocytozoon. The assay takes advantage of a Haemoproteus-specific restriction site identified by sequencing full mitochondrial genomes from two Haemoproteus and three Plasmodium lineages and an adjacent, genus-specific restriction site identified in Leucocytozoon spp. The assay was sensitive to simulated parasitemias of approximately 8 x 10(-6) per erythrocyte and was 100% accurate in differentiating between parasite genera isolated from a broad geographical and taxonomic sampling of infected hosts.Keywords
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