Partial purification and characterization of the human erythrocyte Mg2+ ‐ATPase A candidate aminophospholipid translocase
- 18 June 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 266 (1-2) , 29-32
- https://doi.org/10.1016/0014-5793(90)81498-d
Abstract
A Mg2+-ATPase-enriched fraction was obtained from solubilized human erythrocyte membranes by ammonium sulphate precipitation and anion-exchange chromatography. The solubilized enzyme, of apparent molecular weight 120 kDa, requires phosphatidylserine to be fully active. Phosphatidylethanolamine but not other anionic phospholipids can only partially restore the activity. The Mg-ATPase has a low affinity for Mg2+-ATP and is inhibited by fluoride, vanadate, vanadyl and calcium ions. From these characteristics, we infer that this Mg2+-ATPase is the same protein as the aminophospholipid translocase which regulates the membrane phospholipid transverse distribution in human erythrocytes by actively transporting aminophospholipids from the outer to the inner monolayerKeywords
This publication has 17 references indexed in Scilit:
- Transbilayer movement of phosphatidylserine in nonhuman erythrocytes: evidence that the aminophospholipid transporter is a ubiquitous membrane proteinBiochemistry, 1989
- Control of transmembrane lipid asymmetry in chromaffin granules by an ATP-dependent proteinNature, 1989
- Ion regulation of phosphatidylserine and phosphatidylethanolamine outside-inside translocation in human erythrocytesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1987
- Radioiodinated, photoactivatable phosphatidylcholine and phosphatidylserine: transfer properties and differential photoreactive interaction with human erythrocyte membrane proteinsBiochemistry, 1987
- Outside-inside translocation of aminophospholipids in the human erythrocyte membrane is mediated by a specific enzymeBiochemistry, 1986
- ATP‐dependent translocation of amino phospholipids across the human erythrocyte membraneFEBS Letters, 1986
- Incorporation and translocation of aminophospholipids in human erythrocytesBiochemistry, 1985
- The plasma membrane (Mg2+)-dependent adenosine triphosphatase from the human erythrocyte is not an ion pumpThe Journal of Membrane Biology, 1984
- Activation of (Na+ + K+)-dependent ATPase by lipid vesicles of negative phospholipidsBiochimica et Biophysica Acta (BBA) - Biomembranes, 1977
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970