Leu‐10 (HLA‐DC/DS) antigen distribution in human leukaemic disorders as detected by a monoclonal antibody: correlation with HLA‐DR expression

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Abstract
Summary. Reactivity of the monoclonal antibody anti‐Leu‐10 that detects the human equivalent of the murine 1‐A subregion antigen(s) was studied and correlated with anti‐HLA‐DR expression on 83 cases of acute and chronic leukaemias, leukaemic non‐Hodgkin's lymphomas (NHL) and seven human cell lines. Peripheral blood and/or bone marrow leukaemic cell suspensions were stained by indirect immunofluorescence for both monoclonal antibodies and analysed by flow cytometry. Leu‐10, like HLA‐DR, was absent from T‐cell acute lymphoblastic leukaemia (ALL) (two cases). It was expressed on: 33% of TdT +, CALLA + ALL cases (8/24); 27% (4/15) of acute non‐lymphocytic leukaemia (ANLL); 85% (24/28) of HLA‐DR+B‐cell chronic lymphocytic leukaemia (CLL); and on 9/14 (64%) B‐cell NHL cases. There were no differences in clinical characteristics between Leu‐10+and Leu‐10–patient subgroups. We were able to induce Leu‐10 expression on ‘Josh’ cell line by culturing it with 12‐O‐tetra‐decanoylphorbol‐13‐acetate (TPA). Our data indicate that Leu‐10 expression on leukaemic cells is more restricted than HLA‐DR and is likely to be differentiation related, since it can be induced to be expressed at a later stage than HLA‐DR.