ENZYMATIC DEPHOSPHORYLATION OF OVALBUMIN AND PLAKALBUMIN
Open Access
- 20 May 1952
- journal article
- research article
- Published by Rockefeller University Press in The Journal of general physiology
- Vol. 35 (5) , 711-726
- https://doi.org/10.1085/jgp.35.5.711
Abstract
It has been shown by the work presented in this paper that it is possible to carry out a stepwise dephosphorylation of ovalbumin. With the aid of a prostate phosphatase that attacks only one of the two phosphorus-containing groups in the major component, A1, of ovalbumin, a protein, A2, containing 1 atom of phosphorus per mole has been prepared. Further dephosphorylation with an enzyme of intestinal origin gives a phosphorus-free ovalbumin, A3. Plakalbumin has been similarly dephosphorylated to give P2 and P3. Significant changes in the electrophoretic mobility accompany each dephosphorylation step. This, together with the phosphorus content of the proteins and the crystal form, has been used to characterize and study the five modifications of ovalbumin thus produced.Keywords
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