Release of3H-noradrenaline from the rat vas deferens under various in vitro conditions

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Abstract
The release of 3H-(-)-noradrenaline (NA [norepinephrine]) from rat vas deferens in vitro was examined under various experimental conditions. In normal and reserpinized vas deferens the NA release evoked by (+)-amphetamine (5 .times. 10-6 M) or low external Na+ (26 mM) was antagonized by imipramine methiodide and desipramine, NA uptake inhibitors, but was not dependent on the presence of Ca2+ in the medium and was not antagonized by the potent local anesthetic agent betoxycaine. The release evoked by veratridine in reserpinized tissue was antagonized by the uptake inhibitors but was in normal tissue only partially inhibited in presence of Ca2+ but almost completely in absence of Ca2+. The release by high K+ (117 mM) + low Na+ (26 mM) in normal tissue was dependent on the presence of Ca2+ and was antagonized by the muscarinic agonists carbacholine and methacholine and by high concentrations of desipramine. In the reserpinized vasa the corresponding release was not dependent on Ca2+ and was not antagonized by the muscarinic agents but was inhibited by high concentrations of desipramine.

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