A NEW BIOLOGIC TEST FOR HORMONES IN PREGNANCY URINE

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Abstract
It has been known that male fish of certain species undergo color changes during the breeding season and develop what is known as the "wedding dress." These chromatophore reactions have been developed artificially by stimulation with various hormone preparations.1Following the same principle it was thought that analogous reactions might be obtained on female fish of species that have an externally visible oviduct. Tozawa2found that males of the Japanese bitterling (Acherlognathus intermedium) developed a chromatophore reaction to hormones injected only during the breeding season, April 15 to July 1. In 1932 Fleischmann and Kann3reported that female bitterlings show an enlargement of the ovipositor following injection of an estrogenic preparation (Progynon) in doses of from 40 to 120 mouse units, while physiologic solution of sodium chloride and an anterior pituitary extract yielded no reaction. These authors claim a lengthening of the oviduct with estrogenic substance during

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