Genetic control of the immune response to the terpolymer L‐glutamic acid 60‐L‐alanine 30‐L‐tyrosine10(GAT). III. Restricted heterogeneity of the anti‐GAT response from BALB/c responder mice

Publisher Website
Google Scholar
Abstract
The heterogeneity of the anti‐GAT [terpolymer poly(Glu60, Ala30, Tyr10)] response of GAT responder mice has been analyzed. Purified anti‐GAT antibodies from BALB/c mice belong only to the γ1χ subclass. The isoelectric focusing pattern obtained indicates that the anti‐GAT antibodies are particularly basic and restricted. These results have been confirmed by two‐dimensional polyacrylamide gel electrophoresis; by this technique, we have shown that the γ1 chain and the corresponding χchain of anti‐ GAT antibodies are restricted. All the anti‐GAT antibodies from 10 BALB/c mice bear the cross‐reactive GAT idiotype previously defined (J. Thèze and G. Sommé, Eur. J. Immunol. 1979, 9: 294). The present results indicate that the anti‐GAT repertoire expressed in BALB/c mice is very limited.