METABOLISM OF DIBENZO-PARA-DIOXIN AND CHLORINATED DIBENZO-PARA-DIOXINS BY A BEIJERINCKIA SPECIES

Google Scholar
  • 1 January 1980
    • journal article
    • research article
    • Vol. 39  (2) , 288-296
Abstract
Whole cells of the parent strain of Beijerinckia, grown with succinate and biphenyl, oxidized dibenzo-p-dioxin and several chlorinated dioxins. The rate of oxidation of the chlorinated dibenzo-p-dioxins decreased with an increasing degree of chlorine substitution. A mutant strain (B8/36) of Beijerinckia oxidized dibenzo-p-dioxin to cis-1,2-dihydroxy-1,2-dihydrodibenzo-p-dioxin. The mutant organism also oxidized 2 monochlorinated dibenzo-p-dioxins to cis-dihydrodiols. No metabolites were detected from 2 dichlorinated dibenzo-p-dioxins. Growth of the parent strain of Beijerinckia on succinate was inhibited after 4 h when 0.05% dibenzo-p-dioxin was present in the culture medium. Resting cell suspensions of the parent organism, previously grown with succinate and biphenyl, oxidized dibenzo-p-dioxin to a compound identified as 1,2-dihydroxydibenzo-p-dioxin. Further degradation of this metabolite was not detected, as the compound was a potent mixed-type inhibitor of 2 ring-fission oxygenases present in this organism.