Nα-Ipteroyltetra (γ-Glutamyl)]-Lysine as a Ligand for the Purification of Thymidylate Synthetase by Affinity Chromatography
- 31 December 1977
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 8 (2-3) , 91-98
- https://doi.org/10.1080/00327487808069051
Abstract
Nα[Pteroyltetra (γ-glutamyl)]-lysine Sepharose was synthesized and shown to be a stable, high capacity affinity matrix capable of bringing about the purification of Lactobacillus casei thymidylate synthetase to maximum specific activity from crude extracts in high yield. Under conditions optimal for binding of thymidylate synthetase, dihydrofolate reductase was not bound.Keywords
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