Protective effect of vitamin E on linoleic acid hydroperoxide‐induced injury to human endothelial cells

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Abstract
The protective effects of vitamin E (α‐tocopherol) and its model compounds, which act as antioxidants, on linoleic acid hydroperoxide‐induced injury to human umbilical vein endothelial cells were examined. When incubated at 50 μM with endothelial cells at 37°C for 24 hr, α‐tocopherol protected the cells from injury, and 2,2,5,7,8‐pentamethylchroman‐6‐ol showed a similar protective effect. Trolox C, a water‐soluble vitamin E model compound, had no protective effect. Tocol, a poor antioxidant, proved toxic. During preincubation, α‐tocopherol was incorporated into the cells at 16.6 nmol/ mg protein, while the pentamethylchromanol was incorporated at 0.5 nmol/mg protein; Trolox C was not incorporated at all. The results suggest that agents having both high antioxidant activity and lipophilicity can protect endothelial cells from linoleic acid hydroperoxide‐induced injury.