Heat Labile Tissue α-Globulin and Its Relationship to the Latex Fixation Test

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Abstract
Summary: Saline extracts made from placenta, liver, spleen, lung, heart muscle and kidney appear to contain varying amounts of an α-globulin which denatures at 56°C at 2 hr exposure in the presence of 0.85% NaCl to form macroscopic granules. The reaction is proportional to the concentration of extract used. The addition of polystyrene latex to such a mixture changes the granular state to a floccular one. Albumin and some batches of lyophilized γ-globulin inhibit flocculation. Fresh human serum containing a high antibody titer is the most potent inhibitor of flocculatory activity. Evidence has been presented indicating that the latex fixation test measures the presence in quantity of serum heat labile α-globulin. It has been shown that the addition of lyophilized γ-globulin is unnecessary for the performance of this test. Attention is drawn to the fact that tissue heat labile globulin and the reacting substance in the positive latex fixation test are similar.
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