Abstract
The effect of varying media Ca2+ levels on prolactin synthesis and release was investigated in vitro using the rostral pars distalis of coho salmon (Oncorhynchus kisutch). It was found that media Ca2+ is required for release and that maximum release occurred near physiological levels for plasma-ionized Ca2+ in salmonids. Both higher and lower levels of media Ca2+ were found to release less prolactin.

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