The anaerobic metabolism of citrate in rat liver

Publisher Website
Google Scholar
Abstract
Washed particles of rat liver consumed citrate anaerobically at about 50% of the rate occurring aerobically. The products of the anaerobic reaction were on the average malate plus fumarate 10%, a-oxoglutarate 4%, glutamate 32% and succinate 34%. Other substances found were carbohydrate, lactate and [beta]-hydroxybutyrate. The proportions of the substances formed anaerobically from citrate varied with the amount of tissue added and concentration of di- and tri-phosphopyridine nucleotide and adenosine triphosphate. These 3 substances increased the citrate consumption whereas dithionite, hydroxylamine and ethylene-diaminetetraacetic acid decreased it and cyanide completely abolished it. Glutamic acid was formed from the tissue preparations on incubation without substrate and was also formed from the citrate added as substrate. The anaerobic reactions of citrate are discussed.