Carboxy-Terminal Sequence Determination of Proteins and Peptides with Carboxypeptidase Y

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Abstract
No useful chemical method (similar to that of the Edman degradation) exists that allows a sequence investigation from the carboxy-terminal end of proteins and peptides. Efforts have therefore been centered around the exploitation of enzymatic methods, notably the use of carboxypeptidases for that purpose. Carboxypeptidases are enzymes that remove amino acids one at a time from the carboxy-terminus of a peptide chain.

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