Snake Venom. Protein CM-2 fromBitis arietans(Puff Adder) Venom
- 1 January 1982
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 363 (2) , 1087-1096
- https://doi.org/10.1515/bchm2.1982.363.2.1087
Abstract
Protein CM-2 from B. arietans venom was purified by chromatographic procedures involving Sephadex G-50 and CM-cellulose. The purified protein comprises 82 amino acids including 14 half-cystine residues and its primary structure has been elucidated. Protein CM-2 is not toxic. Although the protein clears a suspension of egg yolk, structural features and the inability to hydrolyse L-.alpha.-lecithin reveal that it cannot be a phospholipase A2. In spite of its sequence showing some homology with that of porcine colipase, protein CM-2 is not a colipase.This publication has 12 references indexed in Scilit:
- Use of fluorescamine as an effective blocking reagent to reduce the background in protein sequence analyses by the beckman automated sequencerAnalytical Biochemistry, 1981
- Purification, Some Properties and Amino‐Acid Sequences of Two Phospholipases A (CM‐II and CM‐III) from Naja naja kaouthia VenomEuropean Journal of Biochemistry, 1980
- Snake Venom ToxinsEuropean Journal of Biochemistry, 1976
- The Amino Acid Sequences of Three Toxins (CM-10, CM-12 and CM-14) fromNaja haje annulifera(Egyptian Cobra) VenomHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1975
- Determination of the Activity of Phospholipases A, C, and DPublished by Wiley ,1974
- Staphylococcal Protease: A Proteolytic Enzyme Specific for Glutamoyl BondsProceedings of the National Academy of Sciences, 1972
- Hydrolysis of Proteins with p-Toluenesulfonic AcidJournal of Biological Chemistry, 1971
- Studies on african snake venoms—I The proteolytic activities of some african viperidae venomsToxicon, 1968
- The action of phospholipase A on lipoproteinsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1965
- Tissue sulfhydryl groupsArchives of Biochemistry and Biophysics, 1959