Reconstitution of Circadian Oscillation of Cyanobacterial KaiC Phosphorylation in Vitro
Top Cited Papers
- 15 April 2005
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 308 (5720) , 414-415
- https://doi.org/10.1126/science.1108451
Abstract
Kai proteins globally regulate circadian gene expression of cyanobacteria. The KaiC phosphorylation cycle, which persists even without transcription or translation, is assumed to be a basic timing process of the circadian clock. We have reconstituted the self-sustainable oscillation of KaiC phosphorylation in vitro by incubating KaiC with KaiA, KaiB, and adenosine triphosphate. The period of the in vitro oscillation was stable despite temperature change (temperature compensation), and the circadian periods observed in vivo in KaiC mutant strains were consistent with those measured in vitro. The enigma of the circadian clock can now be studied in vitro by examining the interactions between three Kai proteins.Keywords
This publication has 15 references indexed in Scilit:
- Reconstitution of Circadian Oscillation of Cyanobacterial KaiC Phosphorylation in VitroScience, 2005
- No Transcription-Translation Feedback in Circadian Rhythm of KaiC PhosphorylationScience, 2005
- The Neurospora Circadian SystemJournal of Biological Rhythms, 2004
- Role of KaiC phosphorylation in the circadian clock system of Synechococcus elongatus PCC 7942Proceedings of the National Academy of Sciences, 2004
- Global gene repression by KaiC as a master process of prokaryotic circadian systemProceedings of the National Academy of Sciences, 2004
- Circadian clock protein KaiC forms ATP-dependent hexameric rings and binds DNAProceedings of the National Academy of Sciences, 2002
- KaiA-stimulated KaiC phosphorylation in circadian timing loops in cyanobacteriaProceedings of the National Academy of Sciences, 2002
- Stopping Time: The Genetics of Fly and Mouse Circadian ClocksAnnual Review of Neuroscience, 2001
- Expression of a Gene Cluster kaiABC as a Circadian Feedback Process in CyanobacteriaScience, 1998
- Circadian rhythms in prokaryotes: luciferase as a reporter of circadian gene expression in cyanobacteria.Proceedings of the National Academy of Sciences, 1993