The Effect of Haematin and Catalase on Streptococcus faecalis var. zymogenes Growing on Glycerol

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Abstract
S. faecalis var. zymogenes was grown aerobically on a complex medium containing glycerol as the C source. Addition of hematin or bovine liver catalase to the growth medium resulted in a small increment in growth yield. Suspensions of bacteria grown in the presence of hematin or catalase, respectively, translocated 0.83-1.98 and 1.33-2.53 protons per oxygen atom consumed in glycerol oxidation. Bacteria grown without hematin or catalase had nil or little respiratory-induced proton translocation during glycerol oxidation. Inclusion of hematin in the growth medium caused the bacterium to form a cyanide- and azide-sensitive catalase. Superoxide dismutase activity was similar whether or not hematin was added to the growth medium.

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